Transcriptome analysis of scions grafted to potato rootstock for improving late blight resistance

Abstract Background Late blight seriously threatens potato cultivation worldwide. The severe and widespread damage caused by the fungal pathogen can lead to drastic decreases in yield. Although grafting technology has been widely used improve crop resistance, effects of on late resistance as well associated molecular mechanisms remain unclear. Therefore, we performed RNA transcriptome sequencing analysis testing scion when blight-resistant variety Qingshu 9 susceptible Favorita were rootstock scion, respectively, vice versa . objective this study was evaluate influence disease clarify related mechanisms. Results A Kyoto Encyclopedia Genes Genomes pathway enrichment revealed that expression levels genes plant–pathogen interactions, plant mitogen-activated protein kinase (MAPK) signaling pathways, hormone signal transduction pathways significantly up-regulated rootstock. Some these encoded calcium-dependent kinases (CDPKs), chitin elicitor receptor (CERKs), LRR serine/threonine (LRR-LRKs), NPR family proteins salicylic acid synthesis pathway, MAPKs which response proteins. When rootstock, only a few upregulated 9. Grafted plants using resistant rootstocks inoculated with P. infestans spores showed significant reductions lesion size while no difference observed We also induction scions, especially scions derived from varieties mediated up-regulation involved scions. Conclusions Our results could render or enhance via up-regulating provide basis for exploring mechanism underlying resistance.